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Flow cytometric measurement of phosphorylated STAT5 in AML: lack of specific association with FLT3 internal tandem duplications.
- Source :
-
Leukemia research [Leuk Res] 2003 Sep; Vol. 27 (9), pp. 803-5. - Publication Year :
- 2003
-
Abstract
- STAT5 phosphorylation has been noted in 69-95% of AML cases by Western blotting. We used flow cytometry to measure phosphorylated STAT5 on a semi-quantitative scale. The method was validated on K562 cells, which constitutively express phosphorylated STAT5, but lose this when BCR-abl tyrosine kinase activity is blocked by STI571. Phosphorylated STAT5 was found to measure 2.22+/-0.09 relative fluorescence units (RFU) falling to 0.925+/-0.005RFU in the presence of STI571. Phosphorylated STAT5 expression was 0.99 to 2.09RFU in 28 primary AML samples. There was no logical cut-off point between positive and negative fluorescence. FLT3 internal tandem duplications, found in 11/28 samples, were not significantly associated with the level of phosphorylated STAT5 expression. We conclude that STAT5 phosphorylation can be measured sensitively by flow cytometry in AML and that its expression should not be dichotomised as present or absent.
- Subjects :
- Acute Disease
Bone Marrow metabolism
Bone Marrow pathology
DNA, Neoplasm analysis
Flow Cytometry
Humans
K562 Cells
Leukemia, Myeloid genetics
Leukemia, Myeloid pathology
Mutation
Phosphorylation
Proto-Oncogene Proteins metabolism
Receptor Protein-Tyrosine Kinases metabolism
Receptors, Cell Surface metabolism
STAT5 Transcription Factor
Tandem Repeat Sequences
fms-Like Tyrosine Kinase 3
DNA-Binding Proteins metabolism
Gene Duplication
Leukemia, Myeloid metabolism
Milk Proteins
Proto-Oncogene Proteins genetics
Receptor Protein-Tyrosine Kinases genetics
Trans-Activators metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0145-2126
- Volume :
- 27
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Leukemia research
- Publication Type :
- Academic Journal
- Accession number :
- 12804638
- Full Text :
- https://doi.org/10.1016/s0145-2126(03)00012-2