Four new cycloartane glycosides from Aquilegia vulgaris and their immunosuppressive activities in mouse allogeneic mixed lymphocyte reaction.

Four new cycloartane glycosides, named aquilegiosides C-F, were isolated from the dried aerial parts of Aquilegia vulgaris. Their structures were determined by two dimensional (2D) NMR spectroscopic analysis and chemical evidence. Aquilegiosides C-F suppressed the proliferation of lymphocytes in mouse allogeneic mixed lymphocyte reaction with IC(50), ranging from 3.7x10(-5) to 2.2x10(-4) M.