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Differential expressions of heme oxygenase-1 gene in CD25- and CD25+ subsets of human CD4+ T cells.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2003 Jul 04; Vol. 306 (3), pp. 701-5. - Publication Year :
- 2003
-
Abstract
- Growing evidence suggests that the immunomodulatory heme oxygenase-1 (HO-1) may have an important role in regulating T-cell responses. In this study, we investigated whether CD4(+)CD25(-) and CD4(+)CD25(+) T cells of human CD4(+) subpopulation could differentially express HO-1. Our results obtained from qualitative reverse transcriptase-polymerase chain reaction and quantitative flow cytometry analyses revealed that the CD4(+)CD25(+) T cells constitutively express HO-1 and that T cell stimulation with plate-bound anti-CD3 in combination with soluble anti-CD28 not only induced HO-1 gene expression in the CD4(+)CD25(-) T cells but also up-regulated HO-1 gene expression in the CD4(+)CD25(+) T cells. Our further studies showed that CD28 signal alone was enough to induce HO-1 expression and CD3 signal, of which signal alone did not induce HO-1 expression, was required at least for full HO-1 expression in both CD25(-) and CD25(+) subsets of human CD4(+) T cells. In addition, transfection of human Jurkat T cells with HO-1 suppressed the cellular proliferation, and this effect was reversed by zinc protoporphyrin, a specific HO competitive inhibitor. Taken together, we have first reported that human CD4(+)CD25(+) regulatory T cells constitutively express HO-1 and that HO-1 inhibits Jurkat T cell proliferation.
- Subjects :
- CD28 Antigens metabolism
CD4-Positive T-Lymphocytes physiology
Cell Division physiology
Gene Expression Regulation, Enzymologic
Heme Oxygenase-1
Humans
Immunomagnetic Separation
Jurkat Cells
Membrane Proteins
CD4-Positive T-Lymphocytes enzymology
Heme Oxygenase (Decyclizing) genetics
Heme Oxygenase (Decyclizing) metabolism
Receptors, Interleukin-2 metabolism
T-Lymphocyte Subsets enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 306
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 12810075
- Full Text :
- https://doi.org/10.1016/s0006-291x(03)01037-4