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Gene expression analysis of murine cells producing amphotropic mouse leukaemia virus at a cultivation temperature of 32 and 37 degrees C.
- Source :
-
The Journal of general virology [J Gen Virol] 2003 Jul; Vol. 84 (Pt 7), pp. 1677-1686. - Publication Year :
- 2003
-
Abstract
- Cultivation of retrovirus packaging cells at 32 degrees C represents a common procedure to achieve high titres in mouse retrovirus production. Gene expression profiling of mouse NIH 3T3 cells producing amphotropic mouse leukaemia virus 4070A revealed that 10 % of the 1176 cellular genes investigated were regulated by temperature shift (37/32 degrees C), while 5 % were affected by retrovirus infection. Strikingly, retrovirus production at 32 degrees C activated the cholesterol biosynthesis/transport pathway and caused an increase in plasma membrane cholesterol levels. Furthermore, these conditions resulted in transcriptional activation of smoothened (smo), patched (ptc) and gli-1; Smo, Ptc and Gli-1, as well as cholesterol, are components of the Sonic hedgehog (Shh) signalling pathway, which directs pattern formation, diversification and tumourigenesis in mammalian cells. These findings suggest a link between cultivation at 32 degrees C, production of MLV-A and the Shh signalling pathway.
- Subjects :
- 3T3 Cells physiology
Animals
Cell Membrane chemistry
Cell Membrane metabolism
Cholesterol analysis
Gene Expression Profiling
Gene Expression Regulation
Hedgehog Proteins
Leukemia Virus, Murine pathogenicity
Mice
Proteins genetics
Signal Transduction
Trans-Activators metabolism
3T3 Cells virology
Leukemia Virus, Murine physiology
Oligonucleotide Array Sequence Analysis
Proteins metabolism
Temperature
Virus Assembly
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1317
- Volume :
- 84
- Issue :
- Pt 7
- Database :
- MEDLINE
- Journal :
- The Journal of general virology
- Publication Type :
- Academic Journal
- Accession number :
- 12810861
- Full Text :
- https://doi.org/10.1099/vir.0.18871-0