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An efficient chemical method for dephosphorylation of phosphopeptides.

Authors :
Kuyama H
Toda C
Watanabe M
Tanaka K
Nishimura O
Source :
Rapid communications in mass spectrometry : RCM [Rapid Commun Mass Spectrom] 2003; Vol. 17 (13), pp. 1493-6.
Publication Year :
2003

Abstract

Phosphate moieties found on serine, threonine or tyrosine residues in peptides, e.g., in proteolytic digests of proteins, were cleaved using hydrofluoric acid or hydrogen fluoride-pyridine without side reactions.<br /> (Copyright 2003 John Wiley & Sons, Ltd.)

Details

Language :
English
ISSN :
0951-4198
Volume :
17
Issue :
13
Database :
MEDLINE
Journal :
Rapid communications in mass spectrometry : RCM
Publication Type :
Academic Journal
Accession number :
12820217
Full Text :
https://doi.org/10.1002/rcm.1078