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[Preliminary study on the molecular mechanism of K562 cell apoptosis induced by As2S2].
- Source :
-
Zhonghua zhong liu za zhi [Chinese journal of oncology] [Zhonghua Zhong Liu Za Zhi] 2003 May; Vol. 25 (3), pp. 220-4. - Publication Year :
- 2003
-
Abstract
- Objective: To investigate the apoptotic inducing effect of As(2)S(2) on K562 cells.<br />Methods: The apoptotic inducing effect of As(2)S(2) on K562 cells was determined by flow cytometry, DNA fragmentation analysis and morphology observation. Expression of protein was determined by Western-blot. RT-PCR was used to evaluate changes in gene expression.<br />Results: Apoptosis of K562 cells was induced by 48 - 72 h exposure to 5 micromol/L As(2)S(2). Apoptosis was induced in (34.4 +/- 3.3)% treated cells by 72 h exposure to 3 micro mol/L As(2)S(2), in (21.8 +/- 3.6)% treated cells by 48 h exposure to 5 micromol/L As(2)S(2) and in (46.0 +/- 5.2)% treated cells by 72 h exposure to As(2)S(2) at the same concentration. With 5 micromol/L As(2)S(2), the protein level of Bcr-Abl and JAK2 decreased, while bax expression was upregulated and c-myc was downregulated both in protein and mRNA level. The activity of caspase 3 in K562 cells was increased by As(2)S(2). As(2)S(2) also induced apoptosis of fresh mononuclear cells derived from chronic myelogenous leukemia (CML) patients.<br />Conclusion: As(2)S(2) can induce apoptosis of CML cells. The decline of Bcr-Abl may play an important role. The upregulation of bax, increase of the activity of caspase 3, downregulation of c-myc and decrease of JAK2 may also be involved in the mechanism.
- Subjects :
- Caspase 3 metabolism
Fusion Proteins, bcr-abl analysis
Humans
Janus Kinase 2 analysis
K562 Cells
Leukemia, Myelogenous, Chronic, BCR-ABL Positive drug therapy
Leukemia, Myelogenous, Chronic, BCR-ABL Positive pathology
bcl-2-Associated X Protein analysis
Apoptosis drug effects
Arsenicals pharmacology
Sulfides pharmacology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0253-3766
- Volume :
- 25
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Zhonghua zhong liu za zhi [Chinese journal of oncology]
- Publication Type :
- Academic Journal
- Accession number :
- 12839680