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Inhibition on telomerase activity and cytotoxic effects by cisplatin in cultured human choroidal melanoma cells.
- Source :
-
Yan ke xue bao = Eye science [Yan Ke Xue Bao] 2003 Mar; Vol. 19 (1), pp. 54-9. - Publication Year :
- 2003
-
Abstract
- Purpose: To study the changes of telomerase activity and cytotoxic effects by Cisplatin; cis-dichlorodiamine platinum (CDDP) in cultured human choroidal melanoma.<br />Material and Methods: The primary cultured human choroidal melanoma cells were cultured in the presence and absence of CDDP with different concentration and time respectively. The toxic effects were evaluated by MTT and the level of telomerase was detected by PCR-ELISA assay. And the relationship between telomerase activity and cytotoxic effects were analyzed by a correlation analysis.<br />Results: Following the increase of the concentration and the time of CDDP, gradually repressed telomerase activity was detected in cultured cells. Meanwhile, the restrain rate of the cells increased. The telomerase activity at 24 h and 1 microgram/ml was repressed significantly compared with the control cells. However, the appearance of cell death lagged behind the decreasing of telomerase.<br />Conclusions: CDDP is an effective telomerase inhibitor in cultured choroidal melanoma cells of human eyes, which presents concentration and time dependency and can cause the death of cultured cells.
- Subjects :
- Adult
Antineoplastic Agents pharmacology
Cell Death drug effects
Choroid Neoplasms enzymology
Female
Humans
Male
Melanoma enzymology
Middle Aged
Telomerase antagonists & inhibitors
Tumor Cells, Cultured
Apoptosis drug effects
Choroid Neoplasms pathology
Cisplatin pharmacology
Melanoma pathology
Telomerase metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1000-4432
- Volume :
- 19
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Yan ke xue bao = Eye science
- Publication Type :
- Academic Journal
- Accession number :
- 12852089