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Kinases, intracellular calcium, and apolipophorin-III influence the adhesion of larval hemocytes of the lepidopterous insect, Galleria mellonella.
- Source :
-
Archives of insect biochemistry and physiology [Arch Insect Biochem Physiol] 2003 Aug; Vol. 53 (4), pp. 158-71. - Publication Year :
- 2003
-
Abstract
- Based on the results from the use of selective inhibitors and activators, active protein kinase A, protein tyrosine kinase, and protein kinase C (PKC) isoforms decreased the adhesion of larval Galleria mellonella hemocytes to glass slides. The protein kinase A inhibitor at all concentrations increased granular cell adhesion only whereas protein tyrosine kinase elevated both granular and plasmatocyte attachment at the lowest concentration. Active, Ca(2+)- and lipid-dependent PKC isoforms limited plasmatocyte and granular cell adhesion whereas PKC that was inhibited by selected compounds (with differed modes of PKC inhibition) enhanced hemocyte attachment. The granular cells were more sensitive to the PKC inhibitors than were plasmatocytes. Phospholipase C and its diacylglyceride product were necessary to reduce hemocyte adhesion and maintain PKC activity. Extracellular Ca(2+), possibly transported through L-channels, was required for plasmatocyte attachment. In contrast, lowering the levels of cytosolic Ca(2+) was associated with decreased PKC activity and was required for hemocyte adhesion.<br /> (Copyright 2003 Wiley-Liss, Inc.)
- Subjects :
- Animals
Apolipoproteins pharmacology
Calcium chemistry
Calcium pharmacology
Cell Adhesion physiology
Diglycerides chemistry
Diglycerides pharmacology
Drug Interactions
Enzyme Activation drug effects
Enzyme Inhibitors pharmacology
Hemocytes cytology
Hemocytes enzymology
Intracellular Fluid chemistry
Isoenzymes antagonists & inhibitors
Isoenzymes metabolism
Isoenzymes pharmacology
Larva metabolism
Lepidoptera enzymology
Phosphotransferases antagonists & inhibitors
Phosphotransferases classification
Phosphotransferases pharmacology
Tetradecanoylphorbol Acetate pharmacology
Type C Phospholipases antagonists & inhibitors
Type C Phospholipases pharmacology
Apolipoproteins metabolism
Calcium metabolism
Hemocytes metabolism
Lepidoptera metabolism
Phosphotransferases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0739-4462
- Volume :
- 53
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Archives of insect biochemistry and physiology
- Publication Type :
- Academic Journal
- Accession number :
- 12886514
- Full Text :
- https://doi.org/10.1002/arch.10097