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Serum-free culture of rhesus monkey embryonic stem cells.
- Source :
-
Archives of andrology [Arch Androl] 2003 Sep-Oct; Vol. 49 (5), pp. 331-42. - Publication Year :
- 2003
-
Abstract
- Previous studies have shown that the maintenance and proliferation of undifferentiated rhesus monkey embryonic stem (rES) cells requires medium supplemented with fetal bovine serum (FBS). Due to the uncharacterized composition and variation in serum nature, the present study aimed to replace the serum-containing medium with a serum-free medium in the rES cell culture. The results showed that after the initial 48-h culture in the routinely used serum-containing medium, rES cells can grow and proliferate for a prolonged period in the serum-free medium composed of DMEM supplemented with a cocktail of BSA, IGF-1, TGF-alpha, bFGF, aFGF, estradiol, and progesterone. rES cells cultured in the serum-free medium maintained high level of alkaline phosphatase activity and OCT4 level. There was no indication of differentiation as judged by the marker gene expression of all three embryonic germ layers and trophoblast. In addition, serum-free culture would not affect the passage capacity and differentiation potential of rES cells. This work will facilitate the future study of induced differentiation of rES cells and other applications.
- Subjects :
- Alkaline Phosphatase metabolism
Animals
Cell Differentiation physiology
Cell Line
Genetic Markers genetics
RNA, Messenger metabolism
Reverse Transcriptase Polymerase Chain Reaction
Totipotent Stem Cells enzymology
Cell Culture Techniques methods
Culture Media, Serum-Free
Macaca mulatta physiology
Totipotent Stem Cells cytology
Subjects
Details
- Language :
- English
- ISSN :
- 0148-5016
- Volume :
- 49
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Archives of andrology
- Publication Type :
- Academic Journal
- Accession number :
- 12893509
- Full Text :
- https://doi.org/10.1080/01485010390204959