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Identification of Thermus thermophilus HB8 tRNA (Gm18) methyltransferase gene.
- Source :
-
Nucleic acids symposium series [Nucleic Acids Symp Ser] 2000 (44), pp. 167-8. - Publication Year :
- 2000
-
Abstract
- For the purpose of identification of the gene for Thermus thermophilus tRNA (Gm18) methyltransferase [tRNA (guanosine-2'-)-methyltransferase, EC 2.1.1.34], the purified enzyme from native source was analyzed by the peptide-mass mapping. The target gene encoded the amino acid sequences of the obtained peptides was searched in data from Thermus thermophilus HB8 genome-sequencing project. We found the target gene AB05130, which was expected to encode a protein composed of 194 amino acid residues and the molecular mass of this protein was calculated as 22083. The recombinant protein was expressed in E. coli as an active form. The Gm18 formation activity of the purified recombinant protein was confirmed by in vitro methylation followed by two-dimensional thin layer chromatography and Liquid Chromatography/Mass Spectrum analysis of substrate tRNA.
- Subjects :
- Base Sequence
Cloning, Molecular
Methylation
Molecular Sequence Data
Molecular Weight
Nucleic Acid Conformation
Peptide Mapping
RNA, Fungal chemistry
RNA, Fungal genetics
RNA, Fungal metabolism
RNA, Transfer, Phe chemistry
RNA, Transfer, Phe genetics
RNA, Transfer, Phe metabolism
Recombinant Proteins chemistry
Recombinant Proteins genetics
Recombinant Proteins metabolism
tRNA Methyltransferases chemistry
tRNA Methyltransferases metabolism
Genes, Bacterial
Thermus thermophilus enzymology
Thermus thermophilus genetics
tRNA Methyltransferases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0261-3166
- Issue :
- 44
- Database :
- MEDLINE
- Journal :
- Nucleic acids symposium series
- Publication Type :
- Academic Journal
- Accession number :
- 12903321
- Full Text :
- https://doi.org/10.1093/nass/44.1.167