[CD45-gating for flow cytometric immunophenotyping of leukemia].

Objective: To establish a reliable and rapid multiparameter flow cytometric approach for leukemia immunophenotyping.
Methods: Leukemia blast cells which exhibit a character of low CD45 expression and low side scatter density are easily recognized by "CD45-SSC gating", we measured 46 patients and 3 normal persons of immunophenotyping by this gating method as well as traditional forwardscatter-side scatter (FSC-SSC) gating method.
Results: 5 of 46 immunophenotypic results from "FSC-SSC gating" showed false diagnosis, which were corrected by "CD45-SSC gating". Further, immunophenotypic mapping of 46 different acute leukemia were obtained by "CD45-SSC gating" and three-color staining, The expression of related antigen could be readily observed.
Conclusions: "CD45-SSC gating" is a reliable and easy method to subclassify leukemia. Three-color staining, which is able to analyze the expression among two related antigens, not only be helpful in leukemia diagnosis but also contribute to study the occurrence, development and prognosis of diseases.