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An effective method for axillary bud culture and RAPD analysis of cloned plants in tetraploid black locust.
- Source :
-
Plant cell reports [Plant Cell Rep] 2003 Oct; Vol. 22 (3), pp. 175-80. Date of Electronic Publication: 2003 Aug 07. - Publication Year :
- 2003
-
Abstract
- An efficient micropropagation technique by axillary bud multiplication was established for cloning tetraploid black locust tree (Robinia pseudoacacia L.). The result showed that the optimal medium for shoot multiplication and elongation was Murashige and Skoog (MS) medium supplemented with 0.5 mg/l 6-benzylaminopurine in combination with 0.5 mg/l kinetin and 0.1 mg/l 1-naphthaleneacetic acid. The best medium for rooting was half-strength MS medium with 0.25 mg/l indole-3-butyric acid. In the present report, we examined the genetic fidelity of the micropropagated plants by the randomly amplified polymorphic DNA (RAPD) method with 25 primers. The cloned plants of tetraploid black locust showed complete stability.
Details
- Language :
- English
- ISSN :
- 0721-7714
- Volume :
- 22
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Plant cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 12904886
- Full Text :
- https://doi.org/10.1007/s00299-003-0661-2