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Evaluation of immune responses of cattle as a means to identify high or low responders and use of a human microarray to differentiate gene expression.
- Source :
-
Genetics, selection, evolution : GSE [Genet Sel Evol] 2003; Vol. 35 Suppl 1, pp. S67-81. - Publication Year :
- 2003
-
Abstract
- An immune response (IR) index to identify cows with high (H) and low (L) antibody-mediated immune responses (AMIR) had been previously devised. High AMIR associated with decreased mastitis and improved response to vaccination. Measurement of cell-mediated immune response (CMIR) was not included in the index; therefore various antigen/adjuvant combinations were evaluated as inducers of DTH to be added to the IR-index. The Bacillus Calmette Guérin (BCG)-induced/purified protein derivative (PPD)-elicited tuberculin skin test is a reliable measure of DTH; however, its use to identify livestock with high CMIR may be confounded due to previous exposure to Mycobacteria tuberculosis. DTH to BCG/PPD was therefore compared with that induced by Mycobacteria phlei (saprophyte) and its derivative phlein as the test antigen. Antibody to OVA was also evaluated. The results indicated that BCG/PPD and M. phlei/phlein induced similar DTH, but cross reaction to PPD was evident following induction of DTH using M. phlei making it a less than ideal alternative for testing livestock. Nonetheless, cows could be ranked for both AMIR and CMIR. RNA from two cows with the highest and lowest IR ranks was then used to probe a human 1.7 kD microarray to determine the ability of a human array to provide information on bovine genes associated with H and L.
- Subjects :
- Animals
Cattle genetics
Gene Expression
Humans
Hypersensitivity, Delayed immunology
Mycobacterium bovis immunology
Mycobacterium phlei immunology
Ovalbumin immunology
Tuberculin immunology
Cattle immunology
Hypersensitivity, Delayed veterinary
Immunity, Cellular immunology
Oligonucleotide Array Sequence Analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0999-193X
- Volume :
- 35 Suppl 1
- Database :
- MEDLINE
- Journal :
- Genetics, selection, evolution : GSE
- Publication Type :
- Academic Journal
- Accession number :
- 12927081
- Full Text :
- https://doi.org/10.1186/1297-9686-35-S1-S67