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[Transduction of plasmid antibiotic resistance determinants with pseudo-T-even bacteriophages].
- Source :
-
Genetika [Genetika] 2003 Jul; Vol. 39 (7), pp. 914-26. - Publication Year :
- 2003
-
Abstract
- Transduction of antibiotic resistance determinants of the plasmid pBR322 with pseudoT-even bacteriophages RB42, RB43, and RB49 was studied. It is established that antibiotic resistance determinants of plasmid pBR322 from Escherichia coli recA(+)- and recA(-)-donor strains do not differ significantly in respect to the efficiency of transduction. Amber mutants RB43-21, RB43-33, and a double amber mutant RB43am21am33 were obtained. These mutants facilitated transduction experiments in some cases. Transduction of antibiotic resistance markers of the vector plasmid pBR325 and recombinant plasmid pVT123, containing a DNA fragment with hoc segE uvsW genes of phage T4, was studied. The frequency of appearance of transductants resistant to pseudoT-even bacteriophages used in transduction was determined, and the sensitivity of resistant transductants to 32 RB bacteriophages and also to phages lambda, T2, T4, T5, T6, T7, and BF23 was estimated. The efficiency of plating pseudoT-even bacteriophages RB42 and RB43 on strain E. coli 802 himA hip carrying mutations in genes that encode subunits of the Integration Host Factor (IHF) was shown to be higher than on isogenic strain E. coli 802. The growth of pseudoT-even bacteriophages limited in vivo by modification-restriction systems of chromosomal (EcoKI, EcoBI), phage (EcoP1I), and plasmid (EcoRI, EcoR124I, and EcoR124II) localization was analyzed. It was shown that these phages were only slightly restricted by the type I modification-restriction systems EcoBI, EcoR124I, and EcoR124II. Phage RB42 was restricted by systems EcoKI, EcoP1I, and EcoRI; phage RB43, by systems EcoKI and EcoRI; and phage RB49, by the EcoRI modification-restriction system.
- Subjects :
- DNA Helicases genetics
DNA Helicases metabolism
DNA Restriction-Modification Enzymes genetics
DNA Restriction-Modification Enzymes metabolism
Deoxyribonuclease EcoRI genetics
Deoxyribonuclease EcoRI metabolism
Endodeoxyribonucleases genetics
Endodeoxyribonucleases metabolism
Escherichia coli genetics
Escherichia coli metabolism
Escherichia coli virology
Escherichia coli Proteins biosynthesis
Escherichia coli Proteins genetics
Genetic Markers
Hydrolases genetics
Hydrolases metabolism
Integration Host Factors biosynthesis
Integration Host Factors genetics
Mutation
Site-Specific DNA-Methyltransferase (Adenine-Specific) genetics
Site-Specific DNA-Methyltransferase (Adenine-Specific) metabolism
T-Phages pathogenicity
Virus Replication
Drug Resistance, Microbial genetics
Plasmids genetics
T-Phages genetics
Transduction, Genetic
Viral Proteins
Subjects
Details
- Language :
- Russian
- ISSN :
- 0016-6758
- Volume :
- 39
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Genetika
- Publication Type :
- Academic Journal
- Accession number :
- 12942776