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Structural basis of a flavivirus recognized by its neutralizing antibody: solution structure of the domain III of the Japanese encephalitis virus envelope protein.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2003 Nov 14; Vol. 278 (46), pp. 46007-13. Date of Electronic Publication: 2003 Sep 02. - Publication Year :
- 2003
-
Abstract
- The flavivirus envelope protein is the dominant antigen in eliciting neutralizing antibodies and plays an important role in inducing immunologic responses in the infected host. We have determined the solution structure of the major antigenic domain (domain III) of the Japanese encephalitis virus (JEV) envelope protein. The JEV domain III forms a beta-barrel type structure composed of six antiparallel beta-strands resembling the immunoglobulin constant domain. We have also identified epitopes of the JEV domain III to its neutralizing antibody by chemical shift perturbation measurements. Site-directed mutagenesis experiments are performed to confirm the NMR results. Our study provides a structural basis for understanding the mechanism of immunologic protection and for rational design of vaccines effective against flaviviruses.
- Subjects :
- Amino Acid Sequence
Antibodies chemistry
Antibodies, Monoclonal
Antigens chemistry
Epitopes
Flavivirus metabolism
Gene Products, env metabolism
Magnetic Resonance Spectroscopy
Models, Molecular
Molecular Sequence Data
Mutagenesis, Site-Directed
Protein Conformation
Protein Structure, Secondary
Protein Structure, Tertiary
Sequence Homology, Amino Acid
Encephalitis Virus, Japanese metabolism
Flavivirus chemistry
Gene Products, env chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 278
- Issue :
- 46
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 12952958
- Full Text :
- https://doi.org/10.1074/jbc.M307776200