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[Study on the interaction between sparfloxacin and serum albumins by fluorescence].
- Source :
-
Guang pu xue yu guang pu fen xi = Guang pu [Guang Pu Xue Yu Guang Pu Fen Xi] 2001 Dec; Vol. 21 (6), pp. 829-32. - Publication Year :
- 2001
-
Abstract
- The binding reaction between sparfloxacion (SPFX) and bovine serum albumin (BSA) or chicken egg albumin (CEA) in aqueous was studied using fluorescence and absorption spectra. Their binding constants are KCEA = 8.29 x 10(6) and KBSA = 4.41 x 10(7), and the binding sites are nCEA = 0.558, nBSA = 0.793 respectively. The action distances (RCEA = 1.99 nm, RBSA = 2.09 nm) and energy transfer efficiencies (ECEA = 0.766, EBSA = 0.714) between donor-acceptor and SPFX were obtained by Förster's nonradiative energy transfer mechanism. The experiment demonstrated that the higher the temperature is, the lower the slopes of quenching curves of BSA and CEA are in presence of different amounts of SPFX. It is confirmed that the combination for SPFX with BSA or CEA is a single static quenching process, their interaction may be interpreted with energy transfer mechanism. Through binding reaction of the two albumins with SPFX, the biological effects and action mechanism of SPFX with albumins in vivo were discussed.
- Subjects :
- Albumins metabolism
Animals
Anti-Infective Agents chemistry
Anti-Infective Agents metabolism
Binding Sites
Cattle
Chickens
Egg Proteins chemistry
Energy Transfer
Humans
Protein Binding
Serum Albumin, Bovine chemistry
Spectrometry, Fluorescence methods
Spectrophotometry, Ultraviolet methods
Egg Proteins metabolism
Fluoroquinolones chemistry
Fluoroquinolones metabolism
Serum Albumin, Bovine metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1000-0593
- Volume :
- 21
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Guang pu xue yu guang pu fen xi = Guang pu
- Publication Type :
- Academic Journal
- Accession number :
- 12958907