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Catalposide protects Neuro 2A cells from hydrogen peroxide-induced cytotoxicity via the expression of heme oxygenase-1.
- Source :
-
Toxicology letters [Toxicol Lett] 2003 Nov 01; Vol. 145 (1), pp. 46-54. - Publication Year :
- 2003
-
Abstract
- Catalposide, the major iridoid glycoside isolated from the stem bark of Catalpa ovata G. Don (Bignoniaceae) has been shown to possess anti-microbial, anti-tumoral, and anti-inflammatory properties. Heme oxygenase-1 (HO-1) is a stress response protein and is known to play a protective role against the oxidative injury. In this study, we examined whether catalposide could protect Neuro 2A cells, a kind of neuronal cell lines, from oxidative damage through the induction of HO-1 protein expression and HO activity. The treatment of the cells with catalposide resulted in dose- and time-dependent up-regulations of both HO-1 protein expression and HO activity. Catalposide protected the cells from hydrogen peroxide-induced cell death. The protective effect of catalposide on hydrogen peroxide-induced cell death was abrogated by zinc protoporphyrin IX (ZnPP IX), a HO inhibitor. Additional experiments revealed the involvement of CO in the cytoprotective effect of catalposide-induced HO-1. These results indicate that catalposide is a potent inducer of HO-1 and HO-1 induction is responsible for the catalposide-mediated cytoprotection against oxidative damage.
- Subjects :
- Animals
Bilirubin metabolism
Blotting, Western
Carbon Monoxide metabolism
Cell Line
Cell Survival drug effects
Heme Oxygenase-1
Iron metabolism
Membrane Proteins
Mice
Oxidative Stress drug effects
Diuretics pharmacology
Glucosides pharmacology
Heme Oxygenase (Decyclizing) biosynthesis
Hydrogen Peroxide antagonists & inhibitors
Hydrogen Peroxide toxicity
Neurons drug effects
Neuroprotective Agents pharmacology
Oxidants toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 0378-4274
- Volume :
- 145
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Toxicology letters
- Publication Type :
- Academic Journal
- Accession number :
- 12962973
- Full Text :
- https://doi.org/10.1016/s0378-4274(03)00268-6