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Novel essential DNA repair proteins Nse1 and Nse2 are subunits of the fission yeast Smc5-Smc6 complex.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2003 Nov 14; Vol. 278 (46), pp. 45460-7. Date of Electronic Publication: 2003 Sep 08. - Publication Year :
- 2003
-
Abstract
- The structural maintenance of chromosomes (SMC) family of proteins play essential roles in genomic stability. SMC heterodimers are required for sister-chromatid cohesion (Cohesin: Smc1 & Smc3), chromatin condensation (Condensin: Smc2 & Smc4), and DNA repair (Smc5 & Smc6). The SMC heterodimers do not function alone and must associate with essential non-SMC subunits. To gain further insight into the essential and DNA repair roles of the Smc5-6 complex, we have purified fission yeast Smc5 and identified by mass spectrometry the co-precipitating proteins, Nse1 and Nse2. We show that both Nse1 and Nse2 interact with Smc5 in vivo, as part of the Smc5-6 complex. Nse1 and Nse2 are essential proteins and conserved from yeast to man. Loss of Nse1 and Nse2 function leads to strikingly similar terminal phenotypes to those observed for Smc5-6 inactivation. In addition, cells expressing hypomorphic alleles of Nse1 and Nse2 are, like Smc5-6 mutants, hypersensitive to DNA damage. Epistasis analysis suggests that like Smc5-6, Nse1, and Nse2 function together with Rhp51 in the homologous recombination repair of DNA double strand breaks. The results of this study strongly suggest that Nse1 and Nse2 are novel non-SMC subunits of the fission yeast Smc5-6 DNA repair complex.
- Subjects :
- Alleles
Amino Acid Sequence
Cell Nucleus metabolism
Chromatin metabolism
Chromatography, Gel
DNA Damage
DNA-Binding Proteins physiology
Dimerization
Dose-Response Relationship, Radiation
Gene Deletion
Mass Spectrometry
Molecular Sequence Data
Mutation
Nuclear Proteins metabolism
Peptides chemistry
Phenotype
Rad51 Recombinase
Recombination, Genetic
Saccharomyces cerevisiae Proteins metabolism
Schizosaccharomyces
Schizosaccharomyces pombe Proteins metabolism
Schizosaccharomyces pombe Proteins physiology
Sequence Homology, Amino Acid
Temperature
Ultraviolet Rays
Cell Cycle Proteins chemistry
DNA Repair
Nuclear Proteins chemistry
Saccharomyces cerevisiae Proteins chemistry
Schizosaccharomyces pombe Proteins chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 278
- Issue :
- 46
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 12966087
- Full Text :
- https://doi.org/10.1074/jbc.M308828200