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Conjugates of PNA with naphthalene diimide derivatives having a broad range of DNA affinities.

Authors :
Mokhir AA
Kraemer R
Source :
Bioconjugate chemistry [Bioconjug Chem] 2003 Sep-Oct; Vol. 14 (5), pp. 877-83.
Publication Year :
2003

Abstract

Peptide nucleic acids (PNAs) are neutral DNA analogues, which bind single-stranded DNA (ssDNA) strongly and with high sequence specificity. However, binding efficiency is dependent on the purine content of the PNA strand. This property make more difficult application of PNA as hybridization probes in, e.g., PNA chips, since at a set temperature the hybridization of a fraction of the DNA targets to the PNA probes does not obey Watson-Crick binding rules. The polypurine PNAs, for example, bind the mismatch containing DNA targets stronger, than the pyrimidine rich PNAs their fully complementary targets. Herein we show that PNA-DNA binding efficiency can be finely tuned by the conjugation of derivatives of naphthalene diimide (NADI) to the N-terminus of PNA using polyamide linkers of different lengths. This approach can potentially be used for the design of PNA probes, which bind their DNA targets with similar affinity independently of the PNA sequence.

Details

Language :
English
ISSN :
1043-1802
Volume :
14
Issue :
5
Database :
MEDLINE
Journal :
Bioconjugate chemistry
Publication Type :
Academic Journal
Accession number :
13129390
Full Text :
https://doi.org/10.1021/bc0256345