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Mutations within the 5' half of the avian retrovirus MC29 v-myc gene alter or abolish transformation of chicken embryo fibroblasts and macrophages.
- Source :
-
Journal of virology [J Virol] 1992 May; Vol. 66 (5), pp. 2698-708. - Publication Year :
- 1992
-
Abstract
- Avian myelocytomatosis virus MC29 induces a wide variety of neoplastic diseases in infected birds and transforms cells of the macrophage lineage as well as fibroblasts and epithelial cells. A biological and biochemical analysis, carried out on a series of in-frame insertion and deletion mutations within the gag-myc gene of MC29, revealed several mutations within the 5' portion of the v-myc gene that encode proteins either completely defective for transformation or compromised in their ability to transform chicken embryo fibroblasts but not macrophages. Mutations within the 3' end of the v-myc gene which disrupt sequences encoding the basic/helix-loop-helix region were defective for transformation of both fibroblasts and macrophages. Eight variants were cloned into the replication-competent avian expression vector RCAS. Analysis of cells infected with transformation-defective, replication-competent viruses confirmed the expression of functionally defective Myc proteins. Further, expression of the transformation defective variant dl91-137 in chicken fibroblasts inhibited subsequent transformation by wild-type MC29. The results reported herein support the hypothesis that Myc proteins function as regulators of transcription in a variety of cell types and clearly point out the necessity of putative regulatory domains within the amino-terminal half of the Myc protein.
- Subjects :
- Animals
Avian Leukosis Virus pathogenicity
Cell Transformation, Viral
Cells, Cultured
Chick Embryo
Cloning, Molecular
DNA Mutational Analysis
Fibroblasts microbiology
Fusion Proteins, gag-onc genetics
Genetic Variation
Genetic Vectors
Macrophages microbiology
Mutation
Oncogene Protein p55(v-myc) biosynthesis
Phenotype
Transcription, Genetic
Transfection
Avian Leukosis Virus genetics
Genes, myc genetics
Oncogene Protein p55(v-myc) genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0022-538X
- Volume :
- 66
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 1313895
- Full Text :
- https://doi.org/10.1128/JVI.66.5.2698-2708.1992