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Herpes simplex virus type 1 gene UL13 encodes a phosphoprotein that is a component of the virion.
- Source :
-
Virology [Virology] 1992 Sep; Vol. 190 (1), pp. 184-92. - Publication Year :
- 1992
-
Abstract
- The UL13 open reading frame of herpes simplex virus type 1 (HSV-1) has been expressed in insect cells by a recombinant baculovirus and in Escherichia coli. In the latter case, the UL13 gene was fused to the gene for glutathione S-transferase (GST) to allow high-level expression of an 80-kDa GST-UL13 fusion protein. Antibody raised against the fusion protein reacted specifically with the 55-kDa UL13 gene product expressed by the recombinant baculovirus. This antibody also recognized a late phosphoprotein in HSV-1-infected cell lysates and a component of purified HSV-1 virions, both with the same electrophoretic mobility as the baculovirus-expressed protein. The virion component was efficiently phosphorylated in vitro by a virion-associated protein kinase. Using the same antibody, the probable homolog of the UL13 gene product was identified in HSV-2-infected cells and purified virions.
- Subjects :
- Animals
Baculoviridae genetics
Blotting, Western
Electrophoresis, Polyacrylamide Gel
Escherichia coli genetics
Kinetics
Phosphoproteins metabolism
Recombinant Proteins genetics
Recombinant Proteins metabolism
Simplexvirus metabolism
Vero Cells
Viral Proteins metabolism
Virion metabolism
Phosphoproteins genetics
Simplexvirus genetics
Viral Proteins genetics
Virion genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0042-6822
- Volume :
- 190
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 1326802
- Full Text :
- https://doi.org/10.1016/0042-6822(92)91204-8