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Detection and partial characterization of proopiomelanocortin-related end-products from the pars intermedia of the toad, Bombina orientalis.
- Source :
-
General and comparative endocrinology [Gen Comp Endocrinol] 1992 Aug; Vol. 87 (2), pp. 197-207. - Publication Year :
- 1992
-
Abstract
- Steady-state analyses were performed on the proopiomelanocortin (POMC)-related end-products present in acid extracts of the pars intermedia of the anuran amphibian, Bombina orientalis. Sephadex G-75 gel filtration chromatography indicated that immunoreactive alpha-MSH-sized material and N-acetylated beta-endorphin-related material are the major POMC-related products present in this tissue. The alpha-MSH-sized immunoreactivity was further fractionated by reversed phase HPLC. The major peak of immunoreactivity isolated by this procedure eluted with the same retention time as synthetic ACTH(1-13)amide. Cation exchange chromatography supported the conclusion that the major storage form of alpha-MSH in the pars intermedia of Bombina is ACTH(1-13)amide. Analysis of Bombina pars intermedia in culture indicated that mono-acetylated and di-acetylated alpha-MSH were the major forms of alpha-MSH secreted into the medium. The major peak of N-acetylated beta-endorphin-related material was further analyzed by cation exchange chromatography and Sephadex G-25 gel filtration column chromatography. The major storage form of beta-endorphin in this tissue is N-acetylated, has a net positive charge at pH 2.75 of +1, and has an apparent molecular weight of 1.2K. The beta-endorphin present in the pars intermedia of this tissue does not undergo further N-acetylation at the time of secretion. These results indicate that in the pars intermedia of the archaeobatrachian, Bombina orientalis, the N-acetylation of alpha-MSH is a cosecretory processing event, whereas N-acetylation of beta-endorphin is a post-translational processing event. These results are compared to other archaeobatrachian and neobatrachian pituitary POMC systems that have been analyzed.
- Subjects :
- Animals
Chromatography, Gel
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Female
Male
Melanocyte-Stimulating Hormones isolation & purification
Peptide Fragments isolation & purification
Protein Processing, Post-Translational
Radioimmunoassay
alpha-MSH biosynthesis
alpha-MSH chemistry
alpha-MSH isolation & purification
beta-Endorphin biosynthesis
beta-Endorphin chemistry
beta-Endorphin isolation & purification
Anura physiology
Melanocyte-Stimulating Hormones biosynthesis
Melanocyte-Stimulating Hormones chemistry
Peptide Fragments biosynthesis
Peptide Fragments chemistry
Pituitary Gland, Anterior metabolism
Pro-Opiomelanocortin metabolism
alpha-MSH analogs & derivatives
beta-Endorphin analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 0016-6480
- Volume :
- 87
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- General and comparative endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 1327951
- Full Text :
- https://doi.org/10.1016/0016-6480(92)90023-d