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Improving purification of recombinant ribonuclease T1.
- Source :
-
Journal of biotechnology [J Biotechnol] 1992 Jun; Vol. 24 (2), pp. 189-94. - Publication Year :
- 1992
-
Abstract
- Purification of recombinant RNase T1 and its mutants has been improved by optimizing bacterial growth conditions, periplasmic fraction preparation and the use of a precolumn. The main part of the chromatographic separation could be automated due to the reproducibility of the procedure.
- Subjects :
- Aspergillus oryzae enzymology
Aspergillus oryzae genetics
Base Sequence
Biotechnology
Chromatography, Ion Exchange
DNA, Fungal genetics
Molecular Sequence Data
Mutagenesis, Site-Directed
Protein Engineering
Recombinant Proteins genetics
Recombinant Proteins isolation & purification
Ribonuclease T1 genetics
Ribonuclease T1 isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 0168-1656
- Volume :
- 24
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 1368355
- Full Text :
- https://doi.org/10.1016/0168-1656(92)90122-p