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Steroid inhibition of neural microvessel morphogenesis in vitro: receptor mediation and astroglial dependence.
- Source :
-
Journal of neurochemistry [J Neurochem] 1992 Mar; Vol. 58 (3), pp. 1023-32. - Publication Year :
- 1992
-
Abstract
- Steroid hormones alter several aspects of microvascular function within the CNS. Both microvessel formation and blood-brain barrier expression appear to be influenced by interactions between astrocytes and endothelial cells. To determine if steroids alter astrocyte-endothelial interactions, we studied their effects on astroglial-induced microvessel morphogenesis in vitro. C6 astroglial cells induce bovine retinal microvascular endothelial cells to differentiate into capillary-like structures. Dexamethasone, hydrocortisone, and progesterone at 10 nM inhibited C6-induced microvessel morphogenesis by 75, 35, and 30%, respectively. Inhibition by dexamethasone was both time and concentration dependent, reaching 80-100% at 1 microM. Tetrahydrocortisone and 17 alpha-hydroxyprogesterone had only marginal inhibitory effects. Cortexolone, a glucocorticoid receptor antagonist, blocked inhibition by dexamethasone. Progesterone receptors were expressed in C6 but not bovine retinal microvascular endothelial cells, identifying the astroglial cell as the likely effector of progesterone-mediated inhibition. Astroglial cells were further implicated as the effectors of steroid-mediated inhibition because none of the steroids inhibited astroglial-independent capillary-like structure formation in response to a reconstituted extracellular matrix, Matrigel. These findings are evidence that steroids modulate neural microvascular endothelial cell functions indirectly through perivascular astrocytes via a receptor-mediated mechanism.
- Subjects :
- Animals
Capillaries
Cell Differentiation
Cells, Cultured
Endothelium, Vascular cytology
Endothelium, Vascular growth & development
Glucocorticoids pharmacology
Receptors, Progesterone metabolism
Retinal Vessels cytology
Astrocytes physiology
Neovascularization, Pathologic prevention & control
Receptors, Steroid physiology
Retinal Vessels growth & development
Steroids pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-3042
- Volume :
- 58
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of neurochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 1371144
- Full Text :
- https://doi.org/10.1111/j.1471-4159.1992.tb09357.x