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Complement activation during storage of blood under normal blood bank conditions. Effects of proteinase inhibitors and leukocyte depletion.
- Source :
-
Blood [Blood] 1992 Jun 01; Vol. 79 (11), pp. 3071-5. - Publication Year :
- 1992
-
Abstract
- During storage of CPD-A1 preserved whole blood factors of the complement cascade become activated, as evidenced by a rapid increase in the concentrations of C3a-desArg and C4a-desArg. After 10 to 14 days of whole blood storage, the elevations of C3a and C4a levels were highly significant. This increase was paralleled by an increase in the concentration of the lysosomal proteinase elastase from polymorphonuclear (PMN) granulocytes. By contrast, the concentration of the C3 activator complex C4b2b remained unchanged even after 3 weeks of storage. The supplementation of the anticoagulant CPD-A1 with the polyvalent-proteinase-inhibitor aprotinin and the specific elastase-inhibitor eglin C failed to inhibit complement activation, whereas leukocyte depletion could partially abolish the increase of the concentration of C4a, but had no effect on C3a concentrations. These observations support the notion that cleavage of C4 during storage of whole blood is partially leukocyte dependent, whereas the activation of C3 is possibly caused by the activation of the alternate pathway of the complement system by contact of plasma with plastic surfaces.
- Subjects :
- Aprotinin pharmacology
Cell Separation
Complement C3a metabolism
Complement C4a metabolism
Humans
Neutrophils enzymology
Pancreatic Elastase blood
Proteins
Serine Proteinase Inhibitors pharmacology
Blood Banks
Blood Preservation
Complement Activation drug effects
Leukocytes physiology
Protease Inhibitors pharmacology
Serpins
Subjects
Details
- Language :
- English
- ISSN :
- 0006-4971
- Volume :
- 79
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Blood
- Publication Type :
- Academic Journal
- Accession number :
- 1375121