Back to Search Start Over

Modulation of hypothalamic norepinephrine release by atrial natriuretic peptide: involvement of cyclic GMP.

Authors :
Giridhar J
Peoples RW
Isom GE
Source :
European journal of pharmacology [Eur J Pharmacol] 1992 Mar 31; Vol. 213 (3), pp. 317-21.
Publication Year :
1992

Abstract

The ability of atrial natriuretic peptide (ANP) to modulate K+-stimulated release of [3H]norepinephrine ([3H]NE) from rat hypothalamic slices was investigated. ANP-(1-28) significantly decreased K+-stimulated [3H]NE release in a concentration-dependent manner (maximal inhibition = 22% of control with 100 nM, ED50 = 70 pM). Pretreatment with pertussis toxin did not alter the response to ANP. 8Br-cGMP (10 microM), a cGMP analog, significantly decreased [3H]NE release and when combined with 10 nM ANP-(1-28), an additive effect was observed. Additionally, 3-isobutyl 1-methylxanthine (IBMX) (200 microM), a phosphodiesterase inhibitor, combined with ANP-(1-28) 10 nM, significantly decreased [3H]NE release. These results indicate that ANP-(1-28) modulated release of [3H]NE from rat hypothalamic slices and the effect is most likely mediated by elevation of intraneuronal cGMP.

Details

Language :
English
ISSN :
0014-2999
Volume :
213
Issue :
3
Database :
MEDLINE
Journal :
European journal of pharmacology
Publication Type :
Academic Journal
Accession number :
1377629
Full Text :
https://doi.org/10.1016/0014-2999(92)90619-f