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Selective generation of antigen-specific human hybridomas optimized for large scale growth in serum-free medium.
- Source :
-
Journal of immunological methods [J Immunol Methods] 1992 Sep 18; Vol. 154 (1), pp. 21-6. - Publication Year :
- 1992
-
Abstract
- The direct propagation of newly formed human hybridomas in serum-free medium selects for hybrids with a metabolism best suited to growth in this environment. Under optimal culture conditions, this procedure results in the generation of antigen-specific human hybridomas comparable in frequency, stability, and antibody secretion rate to that obtained with murine hybridomas. After a transient phase of a few days in the appropriate selection medium supplemented with 1% serum, hybridomas grow in serum-free medium in stationary cultures with a cell doubling time of 15-25 h and an antibody production rate averaging 12 micrograms/10(6) cells/day. Clones propagated in bioreactors exhibited a cell doubling time of 29-35 h and an antibody secretion rate of 10-21 micrograms/10(6) cells/day.
- Subjects :
- Antibodies, Monoclonal biosynthesis
Antibodies, Monoclonal immunology
Antibody Specificity
B-Lymphocytes
Cell Division
Cell Fusion
Cells, Cultured
Clone Cells
Culture Media, Serum-Free
Enzyme-Linked Immunosorbent Assay
Humans
Immunoglobulin G biosynthesis
Immunoglobulin M biosynthesis
Immunoglobulin kappa-Chains biosynthesis
Selection, Genetic
Hybridomas immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1759
- Volume :
- 154
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of immunological methods
- Publication Type :
- Academic Journal
- Accession number :
- 1401940
- Full Text :
- https://doi.org/10.1016/0022-1759(92)90208-b