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Further studies of the distribution of CDF/LIF mRNA.
- Source :
-
Ciba Foundation symposium [Ciba Found Symp] 1992; Vol. 167, pp. 125-35; discussion 135-40. - Publication Year :
- 1992
-
Abstract
- Differentiation choices in the haemopoietic and nervous systems are controlled in part by instructive factors. The cholinergic differentiation factor (CDF, also known as leukaemia inhibitory factor, LIF) affects the development of cultured cells from both systems. To understand the role of CDF/LIF during normal development in vivo, we have begun to localize its mRNA in the late fetal and postnatal rat. Application of reverse transcriptase-polymerase chain reaction and RNase protection methods reveals that CDF/LIF mRNA levels are developmentally modulated in both haemopoietic and neural tissues. A target tissue of cholinergic sympathetic neurons, the footpads that contain the sweat glands, express high levels of this mRNA (relative to mRNA for actin and beta 2-microglobulin). Levels in targets of noradrenergic neurons are lower, but do undergo significant changes during development. Signals are also detected in selective regions of the adult brain, and in embryonic skeletal muscle. This finding in muscle may be significant for motor neurons, because CDF/LIF is a trophic factor for these neurons in culture. Embryonic liver, neonatal thymus and postnatal spleen express CDF/LIF mRNA, and expression in gut is the highest of all tissues examined. The selective tissue distribution and developmental modulation of CDF/LIF mRNA expression support a role for this factor in the normal development of several organ systems.
- Subjects :
- Animals
DNA, Single-Stranded genetics
Immune System growth & development
Leukemia Inhibitory Factor
Nervous System growth & development
Polymerase Chain Reaction
RNA, Messenger isolation & purification
Rats
Ribonucleases
Growth Inhibitors genetics
Immune System embryology
Interleukin-6
Lymphokines genetics
Nervous System embryology
RNA, Messenger metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0300-5208
- Volume :
- 167
- Database :
- MEDLINE
- Journal :
- Ciba Foundation symposium
- Publication Type :
- Academic Journal
- Accession number :
- 1425009
- Full Text :
- https://doi.org/10.1002/9780470514269.ch8