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IL-2-activated CD8+CD44high cells express both adaptive and innate immune system receptors and demonstrate specificity for syngeneic tumor cells.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2003 Oct 01; Vol. 171 (7), pp. 3442-50. - Publication Year :
- 2003
-
Abstract
- CD8(+) T cells depend on the alphabeta TCR for Ag recognition and function. However, Ag-activated CD8(+) T cells can also express receptors of the innate immune system. In this study, we examined the expression of NK receptors on a population of CD8(+) T cells expressing high levels of CD44 (CD8(+)CD44(high) cells) from normal mice. These cells are distinct from conventional memory CD8(+) T cells and they proliferate and become activated in response to IL 2 via a CD48/CD2-dependent mechanism. Before activation, they express low or undetectable levels of NK receptors but upon activation with IL-2 they expressed significant levels of activating NK receptors including 2B4 and NKG2D. Interestingly, the IL-2-activated cells demonstrate a preference in the killing of syngeneic tumor cells. This killing of syngeneic tumor cells was greatly enhanced by the expression of the NKG2D ligand Rae-1 on the target cell. In contrast to conventional CD8(+) T cells, IL-2-activated CD8(+)CD44(high) cells express DAP12, an adaptor molecule that is normally expressed in activated NK cells. These observations indicate that activated CD8(+)CD44(high) cells express receptors of both the adaptive and innate immune system and may play a unique role in the surveillance of host cells that have been altered by infection or transformation.
- Subjects :
- Animals
Antigens, CD physiology
CD2 Antigens physiology
CD48 Antigen
CD8-Positive T-Lymphocytes cytology
CD8-Positive T-Lymphocytes metabolism
Cell Death genetics
Cell Death immunology
Cell Division genetics
Cell Division immunology
Cell Line, Tumor metabolism
Cell Line, Tumor pathology
Cell Line, Tumor transplantation
Cells, Cultured
Immunity, Active genetics
Immunity, Innate genetics
Immunophenotyping
Killer Cells, Natural immunology
Killer Cells, Natural metabolism
Lymphocyte Activation genetics
Lymphoma, T-Cell immunology
Lymphoma, T-Cell pathology
Lymphoma, T-Cell prevention & control
Membrane Proteins biosynthesis
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred DBA
Receptors, KIR
T-Lymphocyte Subsets cytology
T-Lymphocyte Subsets metabolism
CD8-Positive T-Lymphocytes immunology
Cell Line, Tumor immunology
Cytotoxicity, Immunologic genetics
Epitopes, T-Lymphocyte biosynthesis
Hyaluronan Receptors biosynthesis
Interleukin-2 pharmacology
Receptors, Immunologic biosynthesis
T-Lymphocyte Subsets immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 171
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 14500639
- Full Text :
- https://doi.org/10.4049/jimmunol.171.7.3442