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A direct and efficient synthesis method for dumbell-shaped linear DNA using PCR in vitro.

Authors :
Taki M
Kato Y
Miyagishi M
Takagi Y
Sano M
Taira K
Source :
Nucleic acids research. Supplement (2001) [Nucleic Acids Res Suppl] 2003 (3), pp. 191-2.
Publication Year :
2003

Abstract

A linear, covalently-closed, dumbbell-shaped DNA vector including a transcription unit is known to have both biological stability and safety and is expected to be useful for gene therapy. We established an easy, quick, and large preparative synthetic method of modified- and unmodified-dumbbell DNA using an intramolecular cyclization at the DNA termini.

Details

Language :
English
Issue :
3
Database :
MEDLINE
Journal :
Nucleic acids research. Supplement (2001)
Publication Type :
Academic Journal
Accession number :
14510445
Full Text :
https://doi.org/10.1093/nass/3.1.191