Back to Search
Start Over
A direct and efficient synthesis method for dumbell-shaped linear DNA using PCR in vitro.
- Source :
-
Nucleic acids research. Supplement (2001) [Nucleic Acids Res Suppl] 2003 (3), pp. 191-2. - Publication Year :
- 2003
-
Abstract
- A linear, covalently-closed, dumbbell-shaped DNA vector including a transcription unit is known to have both biological stability and safety and is expected to be useful for gene therapy. We established an easy, quick, and large preparative synthetic method of modified- and unmodified-dumbbell DNA using an intramolecular cyclization at the DNA termini.
Details
- Language :
- English
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Nucleic acids research. Supplement (2001)
- Publication Type :
- Academic Journal
- Accession number :
- 14510445
- Full Text :
- https://doi.org/10.1093/nass/3.1.191