Back to Search
Start Over
Coupling gene chip analyses and rat genetic variances in identifying potential target genes that may contribute to neuropathic allodynia development.
- Source :
-
Journal of neurochemistry [J Neurochem] 2003 Nov; Vol. 87 (3), pp. 560-73. - Publication Year :
- 2003
-
Abstract
- Genetic factors and nerve injury-induced changes of gene expression in sensory neurons are potential contributors to tactile allodynia, a neuropathic pain state manifested as hypersensitivity to innocuous mechanical stimulation. To uncover genes relevant to neuropathic allodynia, we analyzed gene expression profiles in dorsal root ganglia (DRG) of spinal nerve-ligated Harlan and Holtzman Sprague Dawley rats, strains with different susceptibilities to neuropathic allodynia. Using Affymetrix gene chips, we identified genes showing differential basal-level expression in these strains without injury-induced regulation. Of more than 8000 genes analyzed, less than 180 genes in each strain were regulated after injury, and 19-22% of that was regulated in a strain-specific manner. Importantly, we identified functionally related genes that were co-regulated post injury in one or both strains. In situ hybridization and real-time PCR analyses of a subset of identified genes confirmed the patterns of the microarray data, and the former also demonstrated that injury-induced changes occurred, not only in neurons, but also in non-neuronal cells. Together, our studies provide a global view of injury plasticity in DRG of these rat stains and support a plasticity-based mechanism mediating variations in allodynia susceptibility, thus providing a source for further characterization of neuropathic pain-relevant genes and potential pathways.
- Subjects :
- Animals
Disease Models, Animal
Disease Progression
Genetic Predisposition to Disease
In Situ Hybridization
Male
Pain Measurement
Polymerase Chain Reaction
Rats
Rats, Inbred Strains
Rats, Sprague-Dawley
Species Specificity
Spinal Nerves injuries
Spinal Nerves physiopathology
Gene Expression Profiling
Genes genetics
Genetic Variation
Neuralgia genetics
Oligonucleotide Array Sequence Analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0022-3042
- Volume :
- 87
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of neurochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 14535940
- Full Text :
- https://doi.org/10.1046/j.1471-4159.2003.02016.x