Back to Search
Start Over
[Effects of D1 and D2 dopamine receptor agonists and antagonists on cerebral ischemia/reperfusion injury].
- Source :
-
Sheng li xue bao : [Acta physiologica Sinica] [Sheng Li Xue Bao] 2003 Oct 25; Vol. 55 (5), pp. 565-70. - Publication Year :
- 2003
-
Abstract
- Gerbil forebrain ischemia/reperfusion(I/R) injury model was used to study the effects of D(1) and D(2) receptor agonists and antagonists on neuronal apoptosis of hippocampal CA1 area. All animals were tested for habituation deficits in an open field test on the 1st, 3rd and 7th days after reperfusion. The animals were then killed, and brains underwent paraffin embedding for hematoxylin-eosin staining, in situ terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick end labeling (TUNEL) staining and immunohistochemistry (bax, bcl-2). The result of open field test showed that the I/R group was significantly impaired (higher activity scores) when compared with the control group. Pretreatment with pergolide significantly reduced this habituation impairment. Forebrain ischemia for 5 min resulted in extensive CA1 apoptosis on the 3rd and 7th days after I/R injury. About 95% neurons in hippocampal CA1 area entered apoptosis and only 2%-7% pyramidal neurons stayed alive due to an inhibition of bcl-2 expression and an increase in bax expression. Pretreatment of pergolide attenuated neuronal damage caused by transient ischemia. Infusion of pergolide could induce the expression of bcl-2 and reduce the expression of bax. Pretreatment with SKF38393, SCH23390 and spiperone had no effects on these changes in this transient I/R injury model. All these results indicate that pergolide plays an important role in the protection of hippocampal neurons from apotosis through upregulating the expression of bcl-2 protein and reducing the expression of bax protein.
- Subjects :
- 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine pharmacology
Animals
Apoptosis
Brain physiopathology
Gerbillinae
Hippocampus physiopathology
Ischemic Attack, Transient physiopathology
Male
Neurons physiology
Pergolide pharmacology
Prosencephalon physiopathology
Proto-Oncogene Proteins biosynthesis
Proto-Oncogene Proteins genetics
Proto-Oncogene Proteins c-bcl-2 biosynthesis
Proto-Oncogene Proteins c-bcl-2 genetics
Receptors, Dopamine D1
Receptors, Dopamine D2
bcl-2-Associated X Protein
Brain Ischemia physiopathology
Dopamine Agonists pharmacology
Dopamine Antagonists pharmacology
Neuroprotective Agents pharmacology
Reperfusion Injury physiopathology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0371-0874
- Volume :
- 55
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Sheng li xue bao : [Acta physiologica Sinica]
- Publication Type :
- Academic Journal
- Accession number :
- 14566405