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Neuroprotective effect of citicoline in 6-hydroxydopamine-lesioned rats and in 6-hydroxydopamine-treated SH-SY5Y human neuroblastoma cells.
- Source :
-
Journal of the neurological sciences [J Neurol Sci] 2003 Nov 15; Vol. 215 (1-2), pp. 105-10. - Publication Year :
- 2003
-
Abstract
- Citicoline (CDP-choline or cytidine 5'-diphosphocholine) has been used as a therapeutic agent in combination with levodopa in the treatment of Parkinson's disease (PD). The present study examines the effects of citicoline by using validated in vivo and in vitro models. Citicoline reduces the cytotoxic effect of 6-hydroxydopamine (6-OHDA)-treated human dopaminergic SH-SY5Y neuroblastoma cells as measured cellular redox activity with 3-[4.5-dimethylthiazol-2-yl]-2.5-diphenyltetrazolium bromide (MTT) and increases the levels of reduced glutathione (GSH), a major antioxidant agent. Moreover, citicoline (500 mg/kg i.p.) administered for 7 days ameliorates functional behaviour by significantly reducing the number of apomorphine-induced contralateral rotations in 6-OHDA rats. Finally, citicoline significantly attenuates substantia nigra (SN) dopaminergic cell dropout and tyrosine hydroxylase immunoreactivity in the ipsilateral striatum in rats injected intrastriatally with 6-hydroxydopamine (6-OHDA).
- Subjects :
- Animals
Antiparkinson Agents therapeutic use
Cell Line, Tumor
Cell Survival drug effects
Cell Survival physiology
Cytidine Diphosphate Choline therapeutic use
Humans
Male
Neuroblastoma drug therapy
Neuroblastoma pathology
Neuroprotective Agents therapeutic use
Rats
Rats, Sprague-Dawley
Antiparkinson Agents pharmacology
Cytidine Diphosphate Choline pharmacology
Neuroblastoma physiopathology
Neuroprotective Agents pharmacology
Oxidopamine toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 0022-510X
- Volume :
- 215
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Journal of the neurological sciences
- Publication Type :
- Academic Journal
- Accession number :
- 14568136
- Full Text :
- https://doi.org/10.1016/s0022-510x(03)00204-1