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Yeast life-span extension by calorie restriction is independent of NAD fluctuation.

Authors :
Anderson RM
Latorre-Esteves M
Neves AR
Lavu S
Medvedik O
Taylor C
Howitz KT
Santos H
Sinclair DA
Source :
Science (New York, N.Y.) [Science] 2003 Dec 19; Vol. 302 (5653), pp. 2124-2126. Date of Electronic Publication: 2003 Nov 06.
Publication Year :
2003

Abstract

Calorie restriction (CR) slows aging in numerous species. In the yeast Saccharomyces cerevisiae, this effect requires Sir2, a conserved NAD+-dependent deacetylase. We report that CR reduces nuclear NAD+ levels in vivo. Moreover, the activity of Sir2 and its human homologue SIRT1 are not affected by physiological alterations in the NAD+:NADH ratio. These data implicate alternate mechanisms of Sir2 regulation by CR.

Details

Language :
English
ISSN :
1095-9203
Volume :
302
Issue :
5653
Database :
MEDLINE
Journal :
Science (New York, N.Y.)
Publication Type :
Academic Journal
Accession number :
14605207
Full Text :
https://doi.org/10.1126/science.1088697