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Direct comparison of steady-state marrow, primed marrow, and mobilized peripheral blood for transduction of hematopoietic stem cells in dogs.
- Source :
-
Human gene therapy [Hum Gene Ther] 2003 Nov 20; Vol. 14 (17), pp. 1683-6. - Publication Year :
- 2003
-
Abstract
- The optimal stem cell source for stem cell gene therapy has not been defined. Most gene transfer studies have used peripheral blood or marrow repopulating cells collected after administration of granulocyte colony-stimulating factor and stem cell factor (G-CSF/SCF). For clinical applications, however, growth factor administration may not be feasible. Thus, in the current study we used a competitive repopulation assay in the dog to directly compare transduction efficiency of steady-state marrow, G-CSF/SCF-primed marrow, and G-CSF/SCF-mobilized peripheral blood. Cells from all three sources were transduced, cryopreserved, and thawed together before infusion into myeloablated dogs. Gene marking in hematopoietic repopulating cells was assessed by polymerase chain reaction. While primed marrow resulted in the highest long-term marking levels, steady-state marrow was transduced at least as efficiently as mobilized peripheral blood in all three dogs. These results suggest that steady-state marrow may be an appropriate source for genetic modification of hematopoietic stem cells.
- Subjects :
- Animals
Antigens, CD34 biosynthesis
Bone Marrow Cells cytology
Dogs
Gene Transfer Techniques
Granulocyte Colony-Stimulating Factor metabolism
Growth Substances metabolism
Hematopoiesis
Hematopoietic Stem Cell Transplantation
Polymerase Chain Reaction
Retroviridae genetics
Stem Cell Factor metabolism
Time Factors
Bone Marrow pathology
Hematopoietic Stem Cell Mobilization
Hematopoietic Stem Cells cytology
Stem Cell Transplantation
Subjects
Details
- Language :
- English
- ISSN :
- 1043-0342
- Volume :
- 14
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- Human gene therapy
- Publication Type :
- Academic Journal
- Accession number :
- 14633410
- Full Text :
- https://doi.org/10.1089/104303403322542329