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[Cloning, expression and purification of SARS coronavirus PUMC2 strain nucleocapsid protein].
- Source :
-
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae [Zhongguo Yi Xue Ke Xue Yuan Xue Bao] 2003 Oct; Vol. 25 (5), pp. 504-7. - Publication Year :
- 2003
-
Abstract
- Objective: To clone, express and purify nucleocapsid protein from SARS coronavirus PUMC2 strain.<br />Methods: According to the published SARS coronavirus genome sequences, the full length cDNA of N protein from SARS coronavirus PUMC2 strain was cloned by RT-PCR and the cDNA was cloned into the pET32a expression vector. The recombinant N protein was expressed in E. coli BL21 (DE3), and purified by Ni(2+)-NTA.<br />Results: Prokaryoticly expressed and purified N protein of SARS coronavirus PUMC2 strain was obtained.<br />Conclusions: The SARS coronavirus recombinant N protein obtained by genetic engineering methods can be used for further functional study of SARS coronavirus N protein.
- Subjects :
- Amino Acid Sequence
Base Sequence
Coronavirus Nucleocapsid Proteins
DNA, Complementary genetics
Escherichia coli genetics
Genetic Vectors
Genome, Viral
Molecular Sequence Data
Nucleocapsid Proteins biosynthesis
Nucleocapsid Proteins isolation & purification
RNA, Viral genetics
Recombinant Fusion Proteins biosynthesis
Recombinant Fusion Proteins genetics
Reverse Transcriptase Polymerase Chain Reaction
Severe acute respiratory syndrome-related coronavirus isolation & purification
Sequence Analysis, DNA
Cloning, Molecular
DNA, Viral genetics
Nucleocapsid Proteins genetics
Severe acute respiratory syndrome-related coronavirus genetics
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1000-503X
- Volume :
- 25
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae
- Publication Type :
- Academic Journal
- Accession number :
- 14650145