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Assessment of in-line near-infrared spectroscopy for continuous monitoring of fermentation processes.
- Source :
-
Biotechnology progress [Biotechnol Prog] 2003 Nov-Dec; Vol. 19 (6), pp. 1816-21. - Publication Year :
- 2003
-
Abstract
- The application of NIR in-line to monitor and control fermentation processes was investigated. Determination of biomass, glucose, and lactic and acetic acids during fermentations of Staphylococcus xylosus ES13 was performed by an interactance fiber optic probe immersed into the culture broth and connected to a NIR instrument. Partial least squares regression (PLSR) calibration models of second derivative NIR spectra in the 700-1800 nm region gave satisfactory predictive models for all parameters of interest: biomass, glucose, and lactic and acetic acids. Batch, repeated batch, and continuous fermentations were monitored and automatically controlled by interfacing the NIR to the bioreactor control unit. The high frequency of data collection permitted an accurate study of the kinetics, supplying lots of data that describe the cultural broth composition and strengthen statistical analysis. Comparison of spectra collected throughout fermentation runs of S. xylosus ES13, Lactobacillus fermentum ES15, and Streptococcus thermophylus ES17 demonstrated the successful extension of a unique calibration model, developed for S. xylosus ES13, to other strains that were differently shaped but growing in the same medium and fermentation conditions. NIR in-line was so versatile as to measure several biochemical parameters of different bacteria by means of slightly adapted models, avoiding a separate calibration for each strain.
- Subjects :
- Bioreactors microbiology
Calibration
Cell Culture Techniques instrumentation
Cell Division physiology
Fermentation physiology
Fiber Optic Technology instrumentation
Lactobacillus cytology
Lactobacillus growth & development
Lactobacillus isolation & purification
Lactobacillus metabolism
Online Systems
Optical Fibers
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Infrared instrumentation
Staphylococcus cytology
Staphylococcus isolation & purification
Streptococcus cytology
Streptococcus growth & development
Streptococcus isolation & purification
Streptococcus metabolism
Acetic Acid analysis
Cell Count methods
Cell Culture Techniques methods
Glucose analysis
Lactic Acid analysis
Spectrophotometry, Infrared methods
Staphylococcus growth & development
Staphylococcus metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 8756-7938
- Volume :
- 19
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Biotechnology progress
- Publication Type :
- Academic Journal
- Accession number :
- 14656161
- Full Text :
- https://doi.org/10.1021/bp034101n