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Toward a functional annotation of the human genome using artificial transcription factors.
- Source :
-
Genome research [Genome Res] 2003 Dec; Vol. 13 (12), pp. 2708-16. - Publication Year :
- 2003
-
Abstract
- We have developed a novel, high-throughput approach to collecting randomly perturbed gene-expression profiles from the human genome.A human 293 cell library that stably expresses randomly chosen zinc-finger transcription factors was constructed, and the expression profile of each cell line was obtained using cDNA microarray technology.Gene expression profiles from a total of 132 cell lines were collected and analyzed by (1) a simple clustering method based on expression-profile similarity, and (2) the shortest-path analysis method. These analyses identified a number of gene groups, and further investigation revealed that the genes that were grouped together had close biological relationships. The artificial transcription factor-based random genome perturbation method thus provides a novel functional genomic tool for annotation and classification of genes in the human genome and those of many other organisms.
- Subjects :
- Antigens, Neoplasm genetics
Antigens, Neoplasm physiology
Cell Line
Computational Biology statistics & numerical data
DNA-Binding Proteins genetics
DNA-Binding Proteins physiology
Gene Expression Profiling methods
Gene Expression Profiling statistics & numerical data
Gene Expression Regulation genetics
Genetic Linkage
Humans
Melanoma genetics
Multigene Family
Oligonucleotide Array Sequence Analysis methods
Oligonucleotide Array Sequence Analysis statistics & numerical data
Recombinant Proteins analysis
Transcription Factors genetics
Zinc Fingers genetics
Computational Biology methods
Genome, Human
Transcription Factors physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1088-9051
- Volume :
- 13
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Genome research
- Publication Type :
- Academic Journal
- Accession number :
- 14656973
- Full Text :
- https://doi.org/10.1101/gr.1397903