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Electrochemical investigation on interaction between DNA with quercetin and Eu-Qu3 complex.
- Source :
-
Journal of inorganic biochemistry [J Inorg Biochem] 2004 Jan; Vol. 98 (1), pp. 79-86. - Publication Year :
- 2004
-
Abstract
- The interactions of quercetin (Qu) and Eu-Qu3 complex with calf thymus DNA were studied using cyclic voltammetry (CV) and double potential step chronocoulometry (DPSCC) at glass carbon electrode (GCE) for the surface method. The method is simple, convenient, reliable, reagent saving. Information such as intrinsic binding constant (K), and binding numbers (n) of bound species per DNA (bp), ratio (K(Ox)/K(Red)) of the binding constants for the oxidized and reduced forms of a bound species and interaction mode was obtained using dsDNA-modified GCE. Quercetin and Eu-Qu3 can both bind to DNA, but quercetin binds to DNA mainly by electrostatic attraction and the complex bind to DNA by both intercalation and electrostatic attraction. For the quercetin/dsDNA-modified GCE systems, a K of (3.80+/-0.3) x 10(4) M(-1), saturation coverage value (Gammas) of (2.28+/-0.2) x 10(-10) mol/cm2 and n of 1.2 were obtained. For the complex system, a saturation coverage value (Gammas) of 1.65 x 10(-10) mol/cm2 and n of 1.8 were obtained.
- Subjects :
- Animals
Carbon
Cattle
Electrochemistry methods
Electrodes
Glass
Kinetics
Organometallic Compounds chemistry
Organometallic Compounds metabolism
Oxidation-Reduction
Spectrophotometry, Ultraviolet
DNA chemistry
DNA metabolism
Europium chemistry
Europium metabolism
Quercetin chemistry
Quercetin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0162-0134
- Volume :
- 98
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of inorganic biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 14659636
- Full Text :
- https://doi.org/10.1016/j.jinorgbio.2003.08.015