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Gc protein-derived macrophage activating factor (GcMAF): isoelectric focusing pattern and tumoricidal activity.
- Source :
-
Anticancer research [Anticancer Res] 2003 Nov-Dec; Vol. 23 (6a), pp. 4451-7. - Publication Year :
- 2003
-
Abstract
- Background: Gc protein is the precursor for Gc protein-derived macrophage activating factor (GcMAF), with three phenotypes: Gc1f, Gc1s and Gc2, based on its electrophoretic mobility. The difference in electrophoretic mobility is because of the difference in its posttranslational sugar moiety composition.<br />Materials and Methods: We compared the difference between Gc protein and GcMAF electrophoretic mobility using the isoelectric focusing (IEF) method. The tumoricidal activity of GcMAF-treated macrophage was evaluated after coculture with L-929 cell. The tumoricidal mechanism was investigated using TNF bioassay and nitric oxide (NO) release.<br />Results: The difference in Gc protein and GcMAF electrophoretic mobility was detected. The tumoricidal activity of GcMAF-treated macrophage was detected, but no release of TNF and NO was detected.<br />Conclusion: The difference of isoelectric focusing mobility in Gc protein and GcMAF would be useful to develop a GcMAF detection method. GcMAF increased macrophage tumoricidal activity but TNF and NO release were not involved in the mechanism.
- Subjects :
- Adult
Aged
Aged, 80 and over
Animals
Cell Line, Tumor
Coculture Techniques
Female
Humans
Isoelectric Focusing
Mice
Mice, Inbred ICR
Middle Aged
Neoplasms, Experimental drug therapy
Neoplasms, Experimental immunology
Neoplasms, Experimental metabolism
Nitric Oxide metabolism
Tumor Necrosis Factor-alpha metabolism
Vitamin D-Binding Protein blood
Immunotherapy, Adoptive methods
Macrophage-Activating Factors analysis
Macrophage-Activating Factors pharmacology
Macrophages drug effects
Macrophages immunology
Vitamin D-Binding Protein analysis
Vitamin D-Binding Protein pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0250-7005
- Volume :
- 23
- Issue :
- 6a
- Database :
- MEDLINE
- Journal :
- Anticancer research
- Publication Type :
- Academic Journal
- Accession number :
- 14666733