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Effects of 15-deoxy-delta12,14-prostaglandin J2 on cell proliferation and apoptosis in ECV304 endothelial cells.
- Source :
-
Acta pharmacologica Sinica [Acta Pharmacol Sin] 2004 Jan; Vol. 25 (1), pp. 47-53. - Publication Year :
- 2004
-
Abstract
- Aim: To investigate the effects of 15-deoxy-delta12,14-prostaglandin J2 (15d-PGJ2) on cell proliferation and apoptosis in ECV304 endothelial cells and related molecular mechanism.<br />Methods: MTT, Hoechst33258, TUNEL, Flow cytometry, DNA ladder, RT-PCR, Western blot, and electrophoretic mobility shift assay (EMSA) analysis were employed.<br />Results: The 15d-PGJ2 induced apoptosis in ECV304 endothelial cells in a dose-dependent manner (the percentage of apoptosis was enhanced from 10.0 %+/-1.3 % to 32.8 %+/-1.6 %), which was accompanied by inhibition of NF-?B and AP-1 DNA binding activity, down-regulation of c-myc, upregulation of Gadd45 and p53, and activation of p38 kinase. However, the expression of p21 was found no significant change.<br />Conclusion: peroxisome proliferator-activated receptor gamma ligand, 15d-PGJ2, can inhibit proliferation and induce apoptosis in ECV304 endothelial cells through different mechanisms.
- Subjects :
- Cell Division drug effects
Cells, Cultured
Humans
Mitogen-Activated Protein Kinases metabolism
NF-kappa B metabolism
Prostaglandin D2 analogs & derivatives
Proto-Oncogene Proteins c-myc metabolism
Transcription Factor AP-1 metabolism
Tumor Suppressor Protein p53 metabolism
Umbilical Cord cytology
p38 Mitogen-Activated Protein Kinases
Apoptosis drug effects
Endothelial Cells metabolism
Prostaglandin D2 pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1671-4083
- Volume :
- 25
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Acta pharmacologica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 14704122