Back to Search
Start Over
The NMR-derived solution structure of a new cationic antimicrobial peptide from the skin secretion of the anuran Hyla punctata.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2004 Mar 26; Vol. 279 (13), pp. 13018-26. Date of Electronic Publication: 2004 Jan 08. - Publication Year :
- 2004
-
Abstract
- Amphibian skin secretions constitute an important source of molecules for antimicrobial drug research in order to combat the increasing resistance of pathogens to conventional antibiotics. Among the various types of substances secreted by the dermal granular amphibian glands, there is a wide range of peptides and proteins, often displaying potent antimicrobial activities and providing an effective defense system against parasite infection. In the present work, we report the NMR solution structure and the biological activity of a cationic 14-residue amphiphilic alpha-helical polypeptide named Hylaseptin P1 (HSP1), isolated from the skin secretion of the hylid frog Hyla punctata. The peptide antimicrobial activity was verified against Candida albicans, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, whereas no significant lytic effect was detected toward red or white blood cells.
- Subjects :
- Amino Acid Sequence
Animals
Candida albicans metabolism
Chromatography, High Pressure Liquid
Circular Dichroism
Dose-Response Relationship, Drug
Erythrocytes drug effects
Escherichia coli metabolism
Ions
Leukocytes drug effects
Magnetic Resonance Spectroscopy
Mass Spectrometry
Microscopy, Atomic Force
Models, Molecular
Molecular Sequence Data
Peptides chemistry
Protein Conformation
Protons
Pseudomonas aeruginosa metabolism
Staphylococcus aureus metabolism
Time Factors
Antimicrobial Cationic Peptides chemistry
Antimicrobial Cationic Peptides pharmacology
Anura metabolism
Skin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 279
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 14715660
- Full Text :
- https://doi.org/10.1074/jbc.M310838200