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[Chemical and chemico-enzymatic synthesis of alpha-thiotriphosphate nucleosides].
- Source :
-
Bioorganicheskaia khimiia [Bioorg Khim] 2003 Nov-Dec; Vol. 29 (6), pp. 616-22. - Publication Year :
- 2003
-
Abstract
- New methods of chemical and chemoenzymatic synthesis of nucleoside 5'-thiophosphates and 5'-alpha-thiotriphosphates are developed. The 5'-alpha-thiotriphosphates are used as substrates both in template-dependent enzymatic PCR synthesis and in a T7-RNA transcription polymerase system. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2003, vol. 29, no. 6; see also http://www.maik.ru.
- Subjects :
- Base Sequence
Electrophoresis, Agar Gel
Nuclear Magnetic Resonance, Biomolecular
Nucleosides chemistry
Nucleosides metabolism
Substrate Specificity
Viral Proteins
DNA-Directed RNA Polymerases metabolism
Nucleosides chemical synthesis
Organophosphorus Compounds chemistry
Sulfhydryl Compounds chemistry
Subjects
Details
- Language :
- Russian
- ISSN :
- 0132-3423
- Volume :
- 29
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Bioorganicheskaia khimiia
- Publication Type :
- Academic Journal
- Accession number :
- 14743536
- Full Text :
- https://doi.org/10.1023/b:rubi.0000008897.08102.ee