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Identification of an antigenic peptide derived from the cancer-testis antigen NY-ESO-1 binding to a broad range of HLA-DR subtypes.
- Source :
-
Cancer immunology, immunotherapy : CII [Cancer Immunol Immunother] 2004 Jul; Vol. 53 (7), pp. 589-99. Date of Electronic Publication: 2004 Jan 23. - Publication Year :
- 2004
-
Abstract
- NY-ESO-1 is a SEREX-defined cancer-testis antigen of which several MHC I, but only few MHC II-restricted epitopes have been identified. Searching for highly promiscuous MHC II-restricted peptides that might be suitable as a CD4+ stimulating vaccine for many patients, we used the SYFPEITHI algorithm and identified an NY-ESO-1-derived pentadecamer epitope (p134-148) that induced specific CD4+ T-cell responses restricted to the HLA-DRB1 subtypes *0101, *0301, *0401, and *0701 that have a cumulative prevalence of 40% in the Caucasian population. The DR restriction of the p134-148 pentadecamer was demonstrated by inhibition with an HLA-DR antibody and a functional peptide displacement titration assay with the pan-DR-binding T-helper epitope PADRE as the competitor. The natural processing and presentation of this epitope was demonstrated by recognition of an NY-ESO-1+ melanoma cell line by T cells with specificity for p134-148. The pentadecamer p134-148 was able to induce CD4+ responses in 4/38 cancer patients tested. However, no strict correlation was found between CD4+ T-cell responses against p134-148 reactivity and anti-NY-ESO-1 antibody titers in the serum of patients, suggesting that CD4+ and B-cell responses are regulated independently. In conclusion, p134-148 holds promise as a broadly applicable peptide vaccine for patients with NY-ESO-1-positive neoplasms.<br /> (Copyright 2004 Springer-Verlag)
- Subjects :
- Antigen-Presenting Cells immunology
Breast Neoplasms immunology
Breast Neoplasms pathology
CD4-Positive T-Lymphocytes immunology
Cells, Cultured
Epitopes
Female
Humans
Lung Neoplasms immunology
Lung Neoplasms pathology
Mesothelioma immunology
Mesothelioma pathology
Pleural Neoplasms immunology
Pleural Neoplasms pathology
Protein Binding
Antigens, Neoplasm immunology
HLA-DR Antigens immunology
Membrane Proteins immunology
Peptide Fragments immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0340-7004
- Volume :
- 53
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Cancer immunology, immunotherapy : CII
- Publication Type :
- Academic Journal
- Accession number :
- 14745515
- Full Text :
- https://doi.org/10.1007/s00262-003-0492-6