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Examination of membrane protein expression in Paracoccus denitrificans by two-dimensional gel electrophoresis.

Authors :
Bouchal P
Kucera I
Source :
Journal of basic microbiology [J Basic Microbiol] 2004; Vol. 44 (1), pp. 17-22.
Publication Year :
2004

Abstract

The well-known metabolic versatility of the soil bacterium Paracoccus denitrificans poses a challenge for modern proteomic approaches. We describe here improved preparation conditions that allow good separation and quantitative analyses of hundreds of membrane or periplasmic proteins. To illustrate this optimized procedure, the results of a screening for membrane proteins associated predominantly with aerobic or anaerobic (denitrifying) modes of growth are presented.

Details

Language :
English
ISSN :
0233-111X
Volume :
44
Issue :
1
Database :
MEDLINE
Journal :
Journal of basic microbiology
Publication Type :
Academic Journal
Accession number :
14768023
Full Text :
https://doi.org/10.1002/jobm.200310312