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Zidovudine-resistant and -sensitive HIV-1 isolates from patients on drug therapy: in vitro studies evaluating level of replication-competent viruses and cytopathogenicity.
- Source :
-
AIDS (London, England) [AIDS] 1992 Dec; Vol. 6 (12), pp. 1445-9. - Publication Year :
- 1992
-
Abstract
- Objective: To compare biological properties of zidovudine-resistant variants of HIV-1 isolated from subjects on long-term drug therapy with drug-sensitive parental isolates obtained from the same patients before initiation of treatment.<br />Methods: Clinical HIV-1 strains were isolated following co-incubation of patient peripheral blood mononuclear cells with mitogen-stimulated umbilical cord blood lymphocytes. Drug resistance was evaluated by infecting MT-4 cells pretreated with zidovudine and maintained under drug pressure.<br />Results: The drug-resistant phenotype remained stable, following many viral replication cycles in the absence of zidovudine. Drug-resistant variants contained fewer replication-competent viruses but were more cytopathogenic than their corresponding zidovudine-sensitive parental strains.<br />Conclusions: These results suggest that drug-resistant strains possess biological properties that may differ from those of drug-sensitive variants of HIV-1.
- Subjects :
- Acquired Immunodeficiency Syndrome drug therapy
Acquired Immunodeficiency Syndrome physiopathology
Cell Survival
Cytopathogenic Effect, Viral
Drug Resistance, Microbial
Genetic Variation
HIV Core Protein p24 analysis
HIV-1 drug effects
Humans
Phenotype
Selection, Genetic
Acquired Immunodeficiency Syndrome microbiology
HIV-1 growth & development
Zidovudine pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0269-9370
- Volume :
- 6
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- AIDS (London, England)
- Publication Type :
- Academic Journal
- Accession number :
- 1492930
- Full Text :
- https://doi.org/10.1097/00002030-199212000-00004