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A novel 110 kDa form of myosin XVIIIA (MysPDZ) is tyrosine-phosphorylated after colony-stimulating factor-1 receptor signalling.
- Source :
-
The Biochemical journal [Biochem J] 2004 May 15; Vol. 380 (Pt 1), pp. 243-53. - Publication Year :
- 2004
-
Abstract
- Macrophage colony-stimulating factor (M-CSF or CSF-1) controls the development of macrophage lineage cells via activation of its tyrosine kinase receptor, c-Fms. After adding CSF-1 to M1 myeloid cells expressing CSF-1R (CSF-1 receptor), tyrosine phosphorylation of many cellular proteins occurs, which might be linked to subsequent macrophage differentiation. The biological significance and characterization of such proteins were explored by a dual strategy comprising two-dimensional SDS/PAGE analysis of cell lysates of CSF-1-treated M1 cells expressing the wild-type or a mutated receptor, together with an enrichment strategy involving a tyrosine-phosphorylated receptor construct. In the present study, we report the identification by MS of a novel, low-abundance, 110 kDa form of myosin XVIIIA (MysPDZ, myosin containing PDZ domain), which appears to be preferentially tyrosine-phosphorylated after CSF-1R activation when compared with other known isoforms. Receptor mutation studies indicate that CSF-1R-dependent tyrosine phosphorylation of p110myosin XVIIIA requires Tyr-559 in the cytoplasmic domain of the receptor and is therefore Src-family kinase-dependent. Gelsolin, Erp61 protein disulphide-isomerase and possibly non-muscle myosin IIA were also tyrosine-phosphorylated under similar conditions. Similar to the more abundant p190 isoform, p110 myosin XVIIIA lacks a PDZ domain and, in addition, it may lack motor activity. The phosphorylation of p110 myosin XVIIIA by CSF-1 may alter its cellular localization or target its association with other proteins.
- Subjects :
- Amino Acid Sequence
Animals
Cell Differentiation
Cell Line drug effects
Cell Line metabolism
Electrophoresis, Gel, Two-Dimensional
Gelsolin metabolism
Genes, fms
Heat-Shock Proteins metabolism
Isomerases metabolism
Macrophages drug effects
Mice
Molecular Sequence Data
Myeloid Cells metabolism
Myosins chemistry
Myosins isolation & purification
Nonmuscle Myosin Type IIA metabolism
Phosphorylation drug effects
Phosphotyrosine analysis
Protein Disulfide-Isomerases
Receptor, Macrophage Colony-Stimulating Factor drug effects
Recombinant Fusion Proteins physiology
Transfection
src-Family Kinases metabolism
Macrophage Colony-Stimulating Factor pharmacology
Myosins metabolism
Protein Processing, Post-Translational drug effects
Receptor, Macrophage Colony-Stimulating Factor physiology
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 1470-8728
- Volume :
- 380
- Issue :
- Pt 1
- Database :
- MEDLINE
- Journal :
- The Biochemical journal
- Publication Type :
- Academic Journal
- Accession number :
- 14969583
- Full Text :
- https://doi.org/10.1042/BJ20031978