Back to Search
Start Over
Non-invasive diagnosis of Helicobacter pylori infection: simplified 13C-urea breath test, stool antigen testing, or DNA PCR in human feces in a clinical laboratory setting?
- Source :
-
Clinical biochemistry [Clin Biochem] 2004 Apr; Vol. 37 (4), pp. 261-7. - Publication Year :
- 2004
-
Abstract
- Objectives: (1) To compare two stool antigen EIAs (HpSA, FemtoLab) and PCR of ureaseA and cagA in feces, with (13)C-urea breath test (UBT). (2) To ascertain whether a simplified UBT (breath collection time = 10 min) is as reliable as the standard assay (30 min).<br />Design and Methods: Helicobacter pylori status was recorded in Group 1 (n = 187) by UBT, H. pylori stool antigen, ureA and cagA PCR in feces. UBT with 10, 20 and 30 min sampling was performed in Group 2 patients (n = 283).<br />Results: The sensitivity and specificity of HpSA, FemtoLab, and ureA were 67% and 99%, 90% and 96%, 35% and 98%, respectively. cagA results were positive in 16/48 H. pylori-positive, and in 5/100 H. pylori-negative patients. The results of UBT with a 10- and 30-min sampling strictly overlapped.<br />Conclusion: UBT with 10 min breath collection and FemtoLab stool antigen assay are the most reliable non-invasive tests to diagnose H. pylori infection.
- Subjects :
- Adult
Aged
Antigens, Bacterial genetics
Antigens, Bacterial immunology
Bacterial Proteins genetics
Carbon Isotopes
DNA, Bacterial genetics
Female
Helicobacter Infections immunology
Helicobacter Infections microbiology
Helicobacter pylori enzymology
Helicobacter pylori genetics
Helicobacter pylori isolation & purification
Humans
Male
Middle Aged
Polymerase Chain Reaction
Sensitivity and Specificity
Urease genetics
Antigens, Bacterial analysis
Breath Tests methods
Clinical Chemistry Tests methods
DNA, Bacterial analysis
Feces microbiology
Helicobacter Infections diagnosis
Urea
Subjects
Details
- Language :
- English
- ISSN :
- 0009-9120
- Volume :
- 37
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Clinical biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 15003727
- Full Text :
- https://doi.org/10.1016/j.clinbiochem.2003.12.004