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Primary cilia of human endothelial cells disassemble under laminar shear stress.
- Source :
-
The Journal of cell biology [J Cell Biol] 2004 Mar 15; Vol. 164 (6), pp. 811-7. - Publication Year :
- 2004
-
Abstract
- We identified primary cilia and centrosomes in cultured human umbilical vein endothelial cells (HUVEC) by antibodies to acetyl-alpha-tubulin and capillary morphogenesis gene-1 product (CMG-1), a human homologue of the intraflagellar transport (IFT) protein IFT-71 in Chlamydomonas. CMG-1 was present in particles along primary cilia of HUVEC at interphase and around the oldest basal body/centriole at interphase and mitosis. To study the response of primary cilia and centrosomes to mechanical stimuli, we exposed cultured HUVEC to laminar shear stress (LSS). Under LSS, all primary cilia disassembled, and centrosomes were deprived of CMG-1. We conclude that the exposure to LSS ends the IFT in cultured endothelial cells.
- Subjects :
- Amino Acid Sequence
Animals
Cell Cycle physiology
Cells, Cultured
Centrosome metabolism
Chlamydomonas cytology
Chlamydomonas genetics
Chlamydomonas metabolism
Cytoskeletal Proteins genetics
Endothelium, Vascular cytology
Flagella metabolism
Humans
Immunohistochemistry
Molecular Sequence Data
Proteins metabolism
Protozoan Proteins genetics
Protozoan Proteins metabolism
Sequence Alignment
Shear Strength
Stress, Mechanical
TRPP Cation Channels
Tubulin chemistry
Tubulin metabolism
Cilia metabolism
Cytoskeletal Proteins metabolism
Endothelial Cells cytology
Endothelial Cells metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9525
- Volume :
- 164
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Journal of cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 15024030
- Full Text :
- https://doi.org/10.1083/jcb.200312133