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Ablation of calcitonin/calcitonin gene-related peptide-alpha impairs fetal magnesium but not calcium homeostasis.
- Source :
-
American journal of physiology. Endocrinology and metabolism [Am J Physiol Endocrinol Metab] 2004 Aug; Vol. 287 (2), pp. E218-26. Date of Electronic Publication: 2004 Mar 23. - Publication Year :
- 2004
-
Abstract
- We used the calcitonin/calcitonin gene-related peptide (CGRP)-alpha gene knockout model (Ct/Cgrp null) to determine whether calcitonin and CGRPalpha are required for normal fetal mineral homeostasis and placental calcium transfer. Heterozygous (Ct/Cgrp(+/-)) and Ct/Cgrp null females were mated to Ct/Cgrp(+/-) males. One or two days before term, blood was collected from mothers and fetuses and analyzed for ionized Ca, Mg, P, parathyroid hormone (PTH), and calcitonin. Amniotic fluid was collected for Ca, Mg, and P. To quantify skeletal mineral content, fetuses were reduced to ash, dissolved in nitric acid, and analyzed by atomic absorption spectroscopy for total Ca and Mg. Placental transfer of (45)Ca at 5 min was assessed. Ct/Cgrp null mothers had significantly fewer viable fetuses in utero compared with Ct/Cgrp(+/-) and wild-type mothers. Fetal serum Ca, P, and PTH did not differ by genotype, but serum Mg was significantly reduced in null fetuses. Placental transfer of (45)Ca at 5 min was normal. The calcium content of the fetal skeleton was normal; however, total Mg content was reduced in Ct/Cgrp null skeletons obtained from Ct/Cgrp null mothers. In summary, maternal absence of calcitonin and CGRPalpha reduced the number of viable fetuses. Fetal absence of calcitonin and CGRPalpha selectively reduced serum and skeletal magnesium content but did not alter ionized calcium, placental calcium transfer, and skeletal calcium content. These findings indicate that calcitonin and CGRPalpha are not needed for normal fetal calcium metabolism but may regulate aspects of fetal Mg metabolism.
- Subjects :
- Amniotic Fluid metabolism
Animals
Calcitonin deficiency
Calcitonin genetics
Calcitonin Gene-Related Peptide deficiency
Calcitonin Gene-Related Peptide genetics
Calcium blood
Embryonic and Fetal Development physiology
Female
Fetal Blood metabolism
Gene Expression Regulation, Developmental
Growth Plate metabolism
Homeostasis genetics
Homeostasis physiology
Litter Size genetics
Litter Size physiology
Magnesium blood
Male
Maternal-Fetal Exchange physiology
Mice
Mice, Knockout
Placenta metabolism
Placentation
Pregnancy
Pregnancy, Animal blood
RNA, Messenger analysis
Calcitonin physiology
Calcitonin Gene-Related Peptide physiology
Calcium metabolism
Fetus metabolism
Magnesium metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0193-1849
- Volume :
- 287
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Endocrinology and metabolism
- Publication Type :
- Academic Journal
- Accession number :
- 15039145
- Full Text :
- https://doi.org/10.1152/ajpendo.00023.2004